《植物生理学报》 2011, 47(3): 269-274

‘巴斗’杏再生体系的建立与耐盐突变体的筛选

杜雪玲^1,*, 余如刚¹, 宋运贤¹, 张振霞²

1淮北师范大学生命科学学院资源植物学安徽省重点实验室, 安徽淮北235000; 2韩山师范学院生物系, 广东潮州521041

通信作者：杜雪玲；E-mail: xuelingdu@yahoo.com.cn；Tel: 0561-3803119

摘　要：

以‘巴斗’杏试管苗茎段为外植体, 研究其再生体系的建立以及在含不同浓度NaCl培养基上诱导耐盐愈伤组织, 筛选 耐盐突变体。结果显示: 茎段在MS+6-BA 1.5 mg•L-1+IBA 0.5 mg•L-1培养基上诱导愈伤组织效果最好, 芽的分化率可达88%; 将出芽愈伤组织块接种到附加IBA 0.5 mg•L-1+KT 2.0 mg•L-1的MS分化培养基上效果最佳, 芽的分化系数最高为12.7; 较理想的生根培养基为MS+NAA 0.1 mg•L-1+IBA 0.2 mg•L-1, 生根率在46.3%以上; 在含0.8% NaCl的愈伤组织诱导培养基 中, 连续继代筛选2代, 转入不含NaCl的分化培养基中, 分化出了完整植株。经继代培养筛选, 测定发现获得的耐盐植株比 正常培养植株的游离脯氨酸含量高。

关键词：‘巴斗’杏; 植株再生; 耐盐筛选; 突变体

收稿：2010-12-16   修定：2011-01-21

资助：2008年度安徽省高校自然科学研究项目(KJ2008B230)。

Establishment of Plant Regeneration System and Salt-Tolerant Mutant Selection of ‘Badou’ Apricot Embryonic Calli

DU Xue-Ling^1,*, YU Ru-Gang¹, SONG Yun-Xian¹, ZHANG Zhen-Xia²

¹College of Life Science and Anhui Key Laboratory of Plant Resources and Biology, Huaibei Normal University, Huaibei, Anhui 235000, China; ²Department of Biology, Hanshan Normal University, Chaozhou, Guangdong 521041, China

Corresponding author: DU Xue-Ling; E-mail: xuelingdu@yahoo.com.cn; Tel: 0561-3803119

Abstract:

Using stem as explant, the regeneration system of ‘Badou’ apricot and filtering of salt tolerance mutant were studied. The results showed that the best callus induction media was on MS media with 6-BA 1.5 mg·L^-1 and IBA 0.5 mg·L^-1, and the rate of differentiation was 88%. The coefficient of differentiation reached 12.7 when the calli were inoculated on the MS media with the IBA 0.5 mg·L^-1 and KT 2.0 mg·L^-1. The most suitable rhizogenic media was MS with NAA 0.1 mg·L^-1 and IBA 0.2 mg·L^-1, and the rooting rate was above 46.3%. The callus could differentiate plant when the calli were inoculated on induction media with 0.8% NaCl. The free proline content of filtering plant was higher than that of the contrast.

Key words: ‘Badou’ apricot; plantlet regeneration; salt-tolerant selection; mutant